TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX124205/GTX124205_41556_20200624_IHC-P_w_23060522_834.webp "TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunoprecipitation of TET2 protein from 293T whole cell extracts using 5 μg of TET2 antibody [N2-2], N-term (GTX124205). Western blot analysis was performed using TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX124205/GTX124205_41556_20170316_IP_w_23060522_933.webp "Immunoprecipitation of TET2 protein from 293T whole cell extracts using 5 μg of TET2 antibody [N2-2], N-term (GTX124205). Western blot analysis was performed using TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.")
![TET2 antibody [N2-2], N-term detects TET2 protein on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded cervical carcinoma. TET2 antibody [N2-2], N-term (GTX124205) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX124205/GTX124205_41466_IHC_2_w_23060522_484.webp "TET2 antibody [N2-2], N-term detects TET2 protein on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded cervical carcinoma. TET2 antibody [N2-2], N-term (GTX124205) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Wild-type (WT) and TET2 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX124205/GTX124205_41466_20190624_WB_KO_watermark_w_23060522_937.webp "Wild-type (WT) and TET2 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX124205/GTX124205_44678_20230728_IHC-P_M_23080901_986.webp "TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX124205/GTX124205_44713_20220617_WB_Fraction_24080602_625.webp "HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX124205/GTX124205_44713_20220916_ICC_IF_24080602_669.webp "TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.")
TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
TET2 antibody [N2-2], N-term
GTX124205
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetTET2
Overview
- SupplierGeneTex
- Product NameTET2 antibody [N2-2], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.78 mg/ml
- ConjugateUnconjugated
- Gene ID54790
- Target nameTET2
- Target descriptiontet methylcytosine dioxygenase 2
- Target synonymsIMD75, KIAA1546, MDS, methylcytosine dioxygenase TET2, probable methylcytosine dioxygenase TET2, ten-eleven translocation 2, tet oncogene family member 2
- HostRabbit
- IsotypeIgG
- Protein IDQ6N021
- Protein NameMethylcytosine dioxygenase TET2
- Scientific DescriptionThe protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. The encoded protein is involved in myelopoiesis, and defects in this gene have been associated with several myeloproliferative disorders. Two variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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![Wild-type (WT) and TET2 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [HL2822] (GTX640093) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640093/GTX640093_T-45355_20240329_WB_KO_watermark_24040123_592.webp)
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![TET2 antibody [N2], N-term detects TET2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TET2 protein stained by TET2 antibody [N2], N-term (GTX124204) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX124204/GTX124204_41458_20160113_IFA_w_23060522_110.webp)
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![TET2 antibody [GT411] detects TET2 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of DDDDK-human TET2-transfected 293T cells 5% SDS-PAGE TET2 antibody [GT411] (GTX629879) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX629879/GTX629879_41526_WB_B_w_23061202_956.webp)
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![TET2 antibody [GT422] immunoprecipitates TET2 protein in IP experiments. IP samples: 30 μg whole cell extract of TET2-transfected 293T cells. A. Control with 5 μg of preimmune Rabbit IgG B. Immunoprecipitation of TET2 protein by 5 μg TET2 antibody [GT422] (GTX629880) 5 % SDS-PAGE The immunoprecipitated TET2 protein was detected by TET2 antibody [GT422] (GTX629880) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX629880/GTX629880_41470_IP_w_23061202_602.webp)
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