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ProductsBack/TFAP2C / AP2 Gamma Antibody (AP)

TFAP2C / AP2 Gamma Antibody (AP)

LS-C409290
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTFAP2C
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TFAP2C / AP2 Gamma Antibody (AP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 31kDa/49kDa, while the observed MW by Western blot was 49kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 31kDa/49kDa, while the observed MW by Western blot was 49kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Alk. Phosphatase
  • Estimated Purity
    ...
  • Gene ID7022
  • Target name
    TFAP2C
  • Target description
    transcription factor AP-2 gamma
  • Target synonyms
    AP2-GAMMA, ERF1, TFAP2G, hAP-2g, transcription factor AP-2 gamma, activating enhancer-binding protein 2 gamma, estrogen receptor factor 1, transcription factor ERF-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AP2 Gamma/TFAP2C using anti-AP2 Gamma/TFAP2C antibody (A01558-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2 Gamma/TFAP2C antigen affinity purified polyclonal antibody (Catalog # A01558-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2 Gamma/TFAP2C at approximately 49 kDa. The expected band size for AP2 Gamma/TFAP2C is at 49 kDa.
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