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Transcription Factor AP 2 gamma Antibody (YA1142, Rabbit)

HY-P81397
MedChem Express
TargetTFAP2C
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Transcription Factor AP 2 gamma Antibody (YA1142, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID7022
  • Target name
    TFAP2C
  • Target description
    transcription factor AP-2 gamma
  • Target synonyms
    AP2-GAMMA, ERF1, TFAP2G, hAP-2g, transcription factor AP-2 gamma, activating enhancer-binding protein 2 gamma, estrogen receptor factor 1, transcription factor ERF-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Transcription Factor AP 2 gamma Antibody (YA1142) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1142), targeting Transcription Factor AP 2 gamma. Transcription Factor AP 2 gamma Antibody (YA1142) can be used for IHC-P experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AP2 Gamma/TFAP2C using anti-AP2 Gamma/TFAP2C antibody (A01558-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2 Gamma/TFAP2C antigen affinity purified polyclonal antibody (Catalog # A01558-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2 Gamma/TFAP2C at approximately 49 kDa. The expected band size for AP2 Gamma/TFAP2C is at 49 kDa.
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