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Western Blot analysis of HeLa cells using TFDP1 Polyclonal Antibody
Western Blot analysis of HeLa cells using TFDP1 Polyclonal Antibody
Western Blot analysis of HeLa cells using TFDP1 Polyclonal Antibody

TFDP1 Antibody

CSB-PA004264
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTFDP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TFDP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7027
  • Target name
    TFDP1
  • Target description
    transcription factor Dp-1
  • Target synonyms
    DILC, DP1, DRTF1, Dp-1, transcription factor Dp-1, DRTF1-polypeptide 1, E2F dimerization partner 1, E2F-related transcription factor, down-regulated in liver cancer stem cells
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14186
  • Protein Name
    Transcription factor Dp-1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DP1 using anti-DP1 antibody (M04204). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DP1 antigen affinity purified monoclonal antibody (Catalog # M04204) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DP1 at approximately 50 kDa. The expected band size for DP1 is at 45 kDa.
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