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ProductsBack/Transcription Factor Dp-1 Recombinant Antbody Kit (KD-Validated)

Transcription Factor Dp-1 Recombinant Antbody Kit (KD-Validated)

BSM-90514R
Bioss
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetTFDP1
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Overview

  • Supplier
    Bioss
  • Product Name
    Transcription Factor Dp-1 Recombinant Antbody Kit (KD-Validated)
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID7027
  • Target name
    TFDP1
  • Target description
    transcription factor Dp-1
  • Target synonyms
    DILC, DP1, DRTF1, Dp-1, transcription factor Dp-1, DRTF1-polypeptide 1, E2F dimerization partner 1, E2F-related transcription factor, down-regulated in liver cancer stem cells
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14186
  • Protein Name
    Transcription factor Dp-1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DP1 using anti-DP1 antibody (M04204). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DP1 antigen affinity purified monoclonal antibody (Catalog # M04204) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DP1 at approximately 50 kDa. The expected band size for DP1 is at 45 kDa.
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