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TFG Polyclonal Antibody

RD84702A
Reddot Biotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetTFG
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    TFG Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10342
  • Target name
    TFG
  • Target description
    trafficking from ER to golgi regulator
  • Target synonyms
    HMSNP, SPG57, TF6, TRKT3, protein TFG, TRK-fused, TRK-fused gene protein, TRKT3 oncogene
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    There are several documented fusion oncoproteins encoded partially by this gene. This gene also participates in several oncogenic rearrangements resulting in anaplastic lymphoma and mixoid chondrosarcoma, and may play a role in the NF-kappaB pathway. Multiple transcript variants have been found for this gene.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRK fused gene/TFG using anti-TRK fused gene/TFG antibody (A02870-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human U-87MG whole cell lysates, Lane 7: human Hacat whole cell lysates, Lane 8: human T-47D whole cell lysates, Lane 9: rat pancreas tissue lysates, Lane 10: rat PC-12 whole cell lysates, Lane 11: mouse small intestine tissue lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRK fused gene/TFG antigen affinity purified polyclonal antibody (Catalog # A02870-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRK fused gene/TFG at approximately 60 kDa. The expected band size for TRK fused gene/TFG is at 43 kDa.
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