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Western Blot Positive WB detected in: Mouse heart tissue, Mouse liver tissue, Mouse kidney tissue All lanes: TGIF1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 30, 32, 28 kDa Observed band size: 44, 50 kDa
Western Blot Positive WB detected in: Mouse heart tissue, Mouse liver tissue, Mouse kidney tissue All lanes: TGIF1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 30, 32, 28 kDa Observed band size: 44, 50 kDa
Western Blot Positive WB detected in: Mouse heart tissue, Mouse liver tissue, Mouse kidney tissue All lanes: TGIF1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 30, 32, 28 kDa Observed band size: 44, 50 kDa

TGIF1 Antibody

CSB-PA623005LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTGIF1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TGIF1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7050
  • Target name
    TGIF1
  • Target description
    TGFB induced factor homeobox 1
  • Target synonyms
    HPE4, TGIF, homeobox protein TGIF1, 5'-TG-3'-interacting factor 1, TALE homeobox TG-interacting factor, transforming growth factor-beta-induced factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15583
  • Protein Name
    Homeobox protein TGIF1
  • Scientific Description
    Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TGIF/TGIF1 using anti-TGIF/TGIF1 antibody (A04122-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGIF/TGIF1 antigen affinity purified polyclonal antibody (Catalog # A04122-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGIF/TGIF1 at approximately 43 kDa. The expected band size for TGIF/TGIF1 is at 43 kDa.
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