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TGIF1 Polyclonal Antibody

CAC14662
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTGIF1
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Overview

  • Supplier
    Biomatik
  • Product Name
    TGIF1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7050
  • Target name
    TGIF1
  • Target description
    TGFB induced factor homeobox 1
  • Target synonyms
    HPE4, TGIF, homeobox protein TGIF1, 5'-TG-3'-interacting factor 1, TALE homeobox TG-interacting factor, transforming growth factor-beta-induced factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15583
  • Protein Name
    Homeobox protein TGIF1
  • Scientific Description
    The TGIF1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Mouse heart tissue, Mouse liver tissue, Mouse kidney tissue All lanes: TGIF1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 30, 32, 28 kDa Observed band size: 44, 50 kDa
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Figure 1. Western blot analysis of TGIF/TGIF1 using anti-TGIF/TGIF1 antibody (A04122-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGIF/TGIF1 antigen affinity purified polyclonal antibody (Catalog # A04122-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGIF/TGIF1 at approximately 43 kDa. The expected band size for TGIF/TGIF1 is at 43 kDa.
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Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
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