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WB analysis of HeLa whole cell lysate using GTX27398 Thymidylate synthase antibody. Dilution : Lane 1 : 1:2000 Lane 2 : 1:5000 Lane 3 : 1:10000 Loading : 4 microg
WB analysis of HeLa whole cell lysate using GTX27398 Thymidylate synthase antibody. Dilution : Lane 1 : 1:2000 Lane 2 : 1:5000 Lane 3 : 1:10000 Loading : 4 microg
WB analysis of HeLa whole cell lysate using GTX27398 Thymidylate synthase antibody. Dilution : Lane 1 : 1:2000 Lane 2 : 1:5000 Lane 3 : 1:10000 Loading : 4 microg

Thymidylate synthase antibody

GTX27398
GeneTex
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetTYMS
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Overview

  • Supplier
    GeneTex
  • Product Name
    Thymidylate synthase antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. ELISA: 1:10000-1:50000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    10 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7298
  • Target name
    TYMS
  • Target description
    thymidylate synthetase
  • Target synonyms
    HST422; thymidylate synthase; TMS; TS; TSase
  • Host
    Sheep
  • Isotype
    IgG
  • Protein IDP04818
  • Protein Name
    Thymidylate synthase
  • Scientific Description
    Because inhibition of Thymidylate Synthase (TS) prevents DNA synthesis and cell proliferation, the enzyme is an important target for cancer chemotherapeutic drugs, specifically the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Thymidylate Synthase/TYMS using anti-Thymidylate Synthase/TYMS antibody (A04320-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thymidylate Synthase/TYMS antigen affinity purified polyclonal antibody (Catalog # A04320-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thymidylate Synthase/TYMS at approximately 36 kDa. The expected band size for Thymidylate Synthase/TYMS is at 36 kDa.
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