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Thymidylate synthase antibody [N3C3]

GTX113289
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetTYMS
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Overview

  • Supplier
    GeneTex
  • Product Name
    Thymidylate synthase antibody [N3C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.53 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7298
  • Target name
    TYMS
  • Target description
    thymidylate synthetase
  • Target synonyms
    HST422; thymidylate synthase; TMS; TS; TSase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04818
  • Protein Name
    Thymidylate synthase
  • Scientific Description
    Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • The Application of Arsenic Trioxide in Ameliorating ABT-737 Target Therapy on Uterine Cervical Cancer Cells through Unique Pathways in Cell Death. Hsin IL et al., 2019 Dec 31, Cancers (Basel)
    Read more
  • Effects and mechanisms of betulinic acid on improving EGFR TKI-resistance of lung cancer cells. Ko JL et al., 2018 Nov, Environ Toxicol
    Read more

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Figure 1. Western blot analysis of Thymidylate Synthase/TYMS using anti-Thymidylate Synthase/TYMS antibody (A04320-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thymidylate Synthase/TYMS antigen affinity purified polyclonal antibody (Catalog # A04320-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thymidylate Synthase/TYMS at approximately 36 kDa. The expected band size for Thymidylate Synthase/TYMS is at 36 kDa.
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