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TLR4 antibody [3C3]

GTX54487
GeneTex
ApplicationsFunctional Assay, Flow Cytometry
Product group Antibodies
TargetTLR4
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Overview

  • Supplier
    GeneTex
  • Product Name
    TLR4 antibody [3C3]
  • Delivery Days Customer
    9
  • Applications
    Functional Assay, Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3C3
  • Concentration
    100 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID7099
  • Target name
    TLR4
  • Target description
    toll like receptor 4
  • Target synonyms
    ARMD10; CD284; homolog of Drosophila toll; hToll; TLR-4; TOLL; toll like receptor 4 protein; toll-like receptor 4
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO00206
  • Protein Name
    Toll-like receptor 4
  • Scientific Description
    The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TLR4 using anti-TLR4 antibody (A00017-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR4 antigen affinity purified polyclonal antibody (Catalog # A00017-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR4 at approximately 100 kDa. The expected band size for TLR4 is at 96 kDa.
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