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TNFRSF17 / BCMA Antibody

LS-C813365
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetTNFRSF17
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TNFRSF17 / BCMA Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID608
  • Target name
    TNFRSF17
  • Target description
    TNF receptor superfamily member 17
  • Target synonyms
    B cell maturation antigen; B-cell maturation factor; B-cell maturation protein; BCM; BCMA; CD269; TNFRSF13A; tumor necrosis factor receptor superfamily member 17
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Raji cells probed with \tBCMA/CD269 Polyclonal Antibody, Unconjugated (bs-3850R) at 1:50 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
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The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA057490(TNFRSF17 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
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Figure 1. Western blot analysis of BCMA using anti-BCMA antibody (A01014-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCMA antigen affinity purified polyclonal antibody (Catalog # A01014-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCMA at approximately 20KD. The expected band size for BCMA is at 20KD.
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