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Western Blot analysis of 293 cells using Tollip Polyclonal Antibody
Western Blot analysis of 293 cells using Tollip Polyclonal Antibody
Western Blot analysis of 293 cells using Tollip Polyclonal Antibody

TOLLIP Antibody

CSB-PA004314
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTOLLIP
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Overview

  • Supplier
    Cusabio
  • Product Name
    TOLLIP Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54472
  • Target name
    TOLLIP
  • Target description
    toll interacting protein
  • Target synonyms
    adapter protein; IL-1RAcPIP; toll-interacting protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H0E2
  • Protein Name
    Toll-interacting protein
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TOLLIP using anti-TOLLIP antibody (A02039-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOLLIP antigen affinity purified polyclonal antibody (Catalog # A02039-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOLLIP at approximately 30 kDa. The expected band size for TOLLIP is at 30 kDa.
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Immunohistochemical staining of human cerebral cortex, epididymis, liver and testis using Anti-TOLLIP antibody HPA038621 (A) shows similar protein distribution across tissues to independent antibody HPA038622 (B).
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