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TOLLIP Antibody

LS-C331982
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetTOLLIP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TOLLIP Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 22kDa/30kDa, while the observed MW by Western blot was 30kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:200), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 22kDa/30kDa, while the observed MW by Western blot was 30kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID54472
  • Target name
    TOLLIP
  • Target description
    toll interacting protein
  • Target synonyms
    adapter protein; IL-1RAcPIP; toll-interacting protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TOLLIP using anti-TOLLIP antibody (A02039-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOLLIP antigen affinity purified polyclonal antibody (Catalog # A02039-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOLLIP at approximately 30 kDa. The expected band size for TOLLIP is at 30 kDa.
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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tollip Polyclonal Antibody, Unconjugated (bs-18168R) at 1:200 overnight at 4°C, DAB staining.
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