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Western blot analysis of extracts of various cell lines using TOMM40 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using TOMM40 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using TOMM40 Polyclonal Antibody at dilution of 1:1000.

TOMM40 Polyclonal Antibody

E-AB-65428
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    TOMM40 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene is localized in the outer membrane of the mitochondria. It is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for import of protein precursors into mitochondria. Alternatively spliced transcript variants have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TOMM40 using anti-TOMM40 antibody (A03166-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOMM40 antigen affinity purified polyclonal antibody (Catalog # A03166-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOMM40 at approximately 38 kDa. The expected band size for TOMM40 is at 38 kDa.
Product group Antibodies
Boster Bio
Anti-TOMM40 Antibody Picoband(r)A03166-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTOMM40
  • SizePrice
Product group Antibodies
RayBiotech
Anti-TOMM40 Antibody144-61359
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetTOMM40
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TOMM40 Polyclonal Antibody, Unconjugated (bs-17227R) at 1:200 overnight at 4°C, DAB staining.
Product group Antibodies
Bioss
TOMM40 Polyclonal AntibodyBS-17227R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rat, Sheep, Xenopus
TargetTOMM40
  • SizePrice
Product group Antibodies
Assay Genie
TOMM40 AntibodyCAB3213
ReactivityHuman
TargetTOMM40
  • SizePrice
Immunohistochemical staining of human cerebral cortex shows weak granular positivity in cytoplasm in neurons.
Product group Antibodies
Atlas Antibodies
Anti-TOMM40 AntibodyHPA036231
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTOMM40
  • SizePrice
Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA024050ESR1HU at dilution of 1:100
Product group Antibodies
Cusabio
TOMM40 AntibodyCSB-PA024050ESR1HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTOMM40
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-TOMM40 AntibodyEB09805
ApplicationsWestern Blot, ELISA
ReactivityBovine, Canine, Human, Mouse, Rat
TargetTOMM40
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
TOMM40 / TOM40 AntibodyLS-C332428
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetTOMM40
  • SizePrice
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TOMM40 antibody (GTX133772) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
TOMM40 antibodyGTX133772
ApplicationsWestern Blot
ReactivityHuman
TargetTOMM40
  • SizePrice
Anti-TOMM40 Antibody
Product group Antibodies
Antibodies.com
Anti-TOMM40 AntibodyA9160
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
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