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Immunofluorescence analysis of U2OS cells using TOX Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using TOX Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using TOX Polyclonal Antibody

TOX Polyclonal Antibody

E-AB-62322
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    TOX Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene contains a HMG box DNA binding domain. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. This protein may function to regulate T-cell development.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TOX using anti-TOX antibody (A08441-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOX antigen affinity purified polyclonal antibody (Catalog # A08441-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOX at approximately 57 kDa. The expected band size for TOX is at 57 kDa.
Product group Antibodies
Boster Bio
Anti-TOX Antibody Picoband(r)A08441-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetTOX
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Product group Antibodies
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Tox Polyclonal AntibodyCAC11318
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetTOX
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Product group Antibodies
Assay Genie
TOX AntibodyCAB7050
ReactivityHuman
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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.
Product group Antibodies
Atlas Antibodies
Anti-TOX AntibodyHPA073241
ApplicationsImmunoCytoChemistry
ReactivityHuman
TargetTOX
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Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate All lanes: TOX antibody at 5.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa
Product group Antibodies
Cusabio
TOX AntibodyCSB-PA024073LA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTOX
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Product group Antibodies
LifeSpan BioSciences
TOX AntibodyLS-C346202
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetTOX
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TOX antibody [N1C1] detects TOX protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. TOX stained by TOX antibody [N1C1] (GTX115287) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Product group Antibodies
GeneTex
TOX antibody [N1C1]GTX115287
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
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Anti-TOX Antibody
Product group Antibodies
Antibodies.com
Anti-TOX AntibodyA10052
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Rat
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