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Transgelin antibody

GTX101608
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTAGLN
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Overview

  • Supplier
    GeneTex
  • Product Name
    Transgelin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.47 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6876
  • Target name
    TAGLN
  • Target description
    transgelin
  • Target synonyms
    SM22, SM22-alpha, SMCC, TAGLN1, TGLN, WS3-10, transgelin, 22 kDa actin-binding protein, epididymis secretory sperm binding protein, smooth muscle protein 22-alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01995
  • Protein Name
    Transgelin
  • Scientific Description
    The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Smith AH, Putta P, Driscoll EC, et al. Canonical transient receptor potential 6 channel deficiency promotes smooth muscle cells dedifferentiation and increased proliferation after arterial injury. JVS Vasc Sci. 2020,1:136-150. doi: 10.1016/j.jvssci.2020.07.002
    Read this paper
  • Stratman AN, Burns MC, Farrelly OM, et al. Chemokine mediated signalling within arteries promotes vascular smooth muscle cell recruitment. Commun Biol. 2020,3(1):734. doi: 10.1038/s42003-020-01462-7
    Read this paper
  • Hao W, Yang R, Yang Y, et al. Stellate ganglion block ameliorates vascular calcification by inhibiting endoplasmic reticulum stress. Life Sci. 2018,193:1-8. doi: 10.1016/j.lfs.2017.12.002
    Read this paper
  • Gautam J, Zhang X, Yao Y. The role of pericytic laminin in blood brain barrier integrity maintenance. Sci Rep. 2016,6:36450. doi: 10.1038/srep36450
    Read this paper
  • Lin LH, Chang SJ, Hu RY, et al. Biomarker discovery for neuroendocrine cervical cancer. Electrophoresis. 2014,35(14):2039-45. doi: 10.1002/elps.201400014
    Read this paper
  • Morita T, Okada M, Yamawaki H. Mechanisms underlying a decrease in KCl-induced contraction after long-term serum-free organ culture of rat isolated mesenteric artery. J Vet Med Sci. 2014,76(7):963-9.
    Read this paper
  • Hampl V, Martin C, Aigner A, et al. Depletion of the transcriptional coactivators megakaryoblastic leukaemia 1 and 2 abolishes hepatocellular carcinoma xenograft growth by inducing oncogene-induced senescence. EMBO Mol Med. 2013,5(9):1367-82. doi: 10.1002/emmm.201202406
    Read this paper

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Figure 1. Western blot analysis of TAGLN/Transgelin using anti-TAGLN/Transgelin antibody (A03962-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat stomach tissue lysates, Lane 3: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAGLN/Transgelin antigen affinity purified polyclonal antibody (Catalog # A03962-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAGLN/Transgelin at approximately 22KD. The expected band size for TAGLN/Transgelin is at 22KD.
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