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TAGLN/Transgelin Antibody (YA1206, Rabbit)

HY-P81461
MedChem Express
TargetTAGLN
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    TAGLN/Transgelin Antibody (YA1206, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6876
  • Target name
    TAGLN
  • Target description
    transgelin
  • Target synonyms
    SM22, SM22-alpha, SMCC, TAGLN1, TGLN, WS3-10, transgelin, 22 kDa actin-binding protein, epididymis secretory sperm binding protein, smooth muscle protein 22-alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    TAGLN/Transgelin Antibody (YA1206) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1206), targeting TAGLN/Transgelin. TAGLN/Transgelin Antibody (YA1206) can be used for IHC-P experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TAGLN/Transgelin using anti-TAGLN/Transgelin antibody (A03962-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat stomach tissue lysates, Lane 3: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAGLN/Transgelin antigen affinity purified polyclonal antibody (Catalog # A03962-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAGLN/Transgelin at approximately 22KD. The expected band size for TAGLN/Transgelin is at 22KD.
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