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Western blot All lanes: TAGLN antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 23 kDa Observed band size: 23 kDa
Western blot All lanes: TAGLN antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 23 kDa Observed band size: 23 kDa
Western blot All lanes: TAGLN antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 23 kDa Observed band size: 23 kDa

TAGLN Antibody

CSB-PA00804A0RB
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTAGLN
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Overview

  • Supplier
    Cusabio
  • Product Name
    TAGLN Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6876
  • Target name
    TAGLN
  • Target description
    transgelin
  • Target synonyms
    22 kDa actin-binding protein; epididymis secretory sperm binding protein; SM22; SM22-alpha; SMCC; smooth muscle protein 22-alpha; TAGLN1; transgelin; transgelin variant 2; WS3-10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01995
  • Protein Name
    Transgelin
  • Scientific Description
    Actin cross-linking/gelling protein By similarity. Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TAGLN/Transgelin using anti-TAGLN/Transgelin antibody (A03962-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat stomach tissue lysates, Lane 3: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAGLN/Transgelin antigen affinity purified polyclonal antibody (Catalog # A03962-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAGLN/Transgelin at approximately 22KD. The expected band size for TAGLN/Transgelin is at 22KD.
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