![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Transglutaminase 2 antibody [N3C3] (GTX111702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Transglutaminase 2 antibody [N3C3] (GTX111702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111702/GTX111702_44524_20211210_WB_22100302_954.webp)
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Transglutaminase 2 antibody [N3C3] (GTX111702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Transglutaminase 2 antibody [N3C3]
GTX111702
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTGM2
Overview
- SupplierGeneTex
- Product NameTransglutaminase 2 antibody [N3C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.05 mg/ml
- ConjugateUnconjugated
- Gene ID7052
- Target nameTGM2
- Target descriptiontransglutaminase 2
- Target synonymsG(h), TG(C), TGC, hTG2, tTG, protein-glutamine gamma-glutamyltransferase 2, C polypeptide, protein-glutamine-gamma-glutamyltransferase, TGase C, TGase H, TGase II, TGase-2, erythrocyte transglutaminase, heart G alpha(h), hhG alpha(h), isopeptidase TGM2, protein G alpha(h), protein-glutamine deamidase TGM2, protein-glutamine dopaminyltransferase TGM2, protein-glutamine histaminyltransferase TGM2, protein-glutamine noradrenalinyltransferase TGM2, protein-glutamine serotonyltransferase TGM2, tTgase, tissue transglutaminase, transglutaminase C, transglutaminase H, transglutaminase II
- HostRabbit
- IsotypeIgG
- Protein IDP21980
- Protein NameProtein-glutamine gamma-glutamyltransferase 2
- Scientific DescriptionTransglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Cao Q, Tartaglia G, Alexander M, et al. Collagen VII maintains proteostasis in dermal fibroblasts by scaffolding TANGO1 cargo. Matrix Biol. 2022,111:226-244. doi: 10.1016/j.matbio.2022.06.008Read this paper
- Xu P, Chen C, Zhang Y, et al. Erythrocyte transglutaminase-2 combats hypoxia and chronic kidney disease by promoting oxygen delivery and carnitine homeostasis. Cell Metab. 2022,34(2):299-316.e6. doi: 10.1016/j.cmet.2021.12.019Read this paper
- Morotomi-Yano K, Yano KI. Calcium-dependent activation of transglutaminase 2 by nanosecond pulsed electric fields. FEBS Open Bio. 2017,7(7):934-943. doi: 10.1002/2211-5463.12227Read this paper
- Yeo SY, Itahana Y, Guo AK, et al. Transglutaminase 2 contributes to a TP53-induced autophagy program to prevent oncogenic transformation. Elife. 2016,5:e07101. doi: 10.7554/eLife.07101Read this paper
- Costa-Almeida R, Gomez-Lazaro M, Ramalho C, et al. Fibroblast-endothelial partners for vascularization strategies in tissue engineering. Tissue Eng Part A. 2015,21(5-6):1055-65. doi: 10.1089/ten.TEA.2014.0443Read this paper
![Transglutaminase 2 antibody [N3C3] detects Transglutaminase 2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Transglutaminase 2 protein stained by Transglutaminase 2 antibody [N3C3] (GTX111702) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000. Blue: Hoechst 33342 staining. Transglutaminase 2 antibody [N3C3] detects Transglutaminase 2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Transglutaminase 2 protein stained by Transglutaminase 2 antibody [N3C3] (GTX111702) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111702/GTX111702_40065_20150410_IFA_w_23060500_864.webp)
![Transglutaminase 2 antibody [N3C3] detects Transglutaminase 2 protein at cytoplasm on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Transglutaminase 2 antibody [N3C3] (GTX111702) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min Transglutaminase 2 antibody [N3C3] detects Transglutaminase 2 protein at cytoplasm on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Transglutaminase 2 antibody [N3C3] (GTX111702) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX111702/GTX111702_40065_20150226_IHC_M_w_23060500_881.webp)

![Transglutaminase 2 antibody [N3C3] detects Transglutaminase 2 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Transglutaminase 2 stained by Transglutaminase 2 antibody [N3C3] (GTX111702) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Transglutaminase 2 antibody [N3C3] detects Transglutaminase 2 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Transglutaminase 2 stained by Transglutaminase 2 antibody [N3C3] (GTX111702) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX111702/GTX111702_44524_20230616_IHC-P_M_23062718_709.webp)
![Transglutaminase 2 antibody [N3C3] detects Transglutaminase 2 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Transglutaminase 2 stained by Transglutaminase 2 antibody [N3C3] (GTX111702) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Transglutaminase 2 antibody [N3C3] detects Transglutaminase 2 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Transglutaminase 2 stained by Transglutaminase 2 antibody [N3C3] (GTX111702) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX111702/GTX111702_44524_20230616_IHC-P_R_23062718_394.webp)









