Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody [HL3809] (GTX642033) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody [HL3809] (GTX642033) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX642033/GTX642033_T-45691_20250221_WB_M_R_25022521_504.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody [HL3809] (GTX642033) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Wild-type (WT) and TRAP1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody [HL3809] (GTX642033) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX642033/GTX642033_T-45691_20250228_WB_KO_watermark_25030600_539.webp "Wild-type (WT) and TRAP1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody [HL3809] (GTX642033) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody [HL3809] (GTX642033) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX642033/GTX642033_T-45691_20250228_WB_R_brain_25030600_784.webp "Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody [HL3809] (GTX642033) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![TRAP1 antibody [HL3809] detects TRAP1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: TRAP1 stained by TRAP1 antibody [HL3809] (GTX642033) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX642033/GTX642033_T-45691_20250328_ICC_IF_25042920_545.webp "TRAP1 antibody [HL3809] detects TRAP1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: TRAP1 stained by TRAP1 antibody [HL3809] (GTX642033) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody [HL3809] (GTX642033) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
TRAP1 antibody [HL3809]
GTX642033
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTRAP1
Overview
- SupplierGeneTex
- Product NameTRAP1 antibody [HL3809]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3809
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID10131
- Target nameTRAP1
- Target descriptionTNF receptor associated protein 1
- Target synonymsHSP 75, HSP75, HSP90L, TRAP-1, heat shock protein 75 kDa, mitochondrial, TNFR-associated protein 1, heat shock protein family C member 5, testicular tissue protein Li 209, tumor necrosis factor type 1 receptor-associated protein
- HostRabbit
- IsotypeIgG
- Protein IDQ12931
- Protein NameHeat shock protein 75 kDa, mitochondrial
- Scientific DescriptionThis gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
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