
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE GTX115352 diluted at 1:3000
TRAP1 antibody [N1N3]
GTX115352
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTRAP1
Overview
- SupplierGeneTex
- Product NameTRAP1 antibody [N1N3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.9 mg/ml
- ConjugateUnconjugated
- Gene ID10131
- Target nameTRAP1
- Target descriptionTNF receptor associated protein 1
- Target synonymsHSP 75, HSP75, HSP90L, TRAP-1, heat shock protein 75 kDa, mitochondrial, TNFR-associated protein 1, heat shock protein family C member 5, testicular tissue protein Li 209, tumor necrosis factor type 1 receptor-associated protein
- HostRabbit
- IsotypeIgG
- Protein IDQ12931
- Protein NameHeat shock protein 75 kDa, mitochondrial
- Scientific DescriptionHSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. TRAP1 is a mitochondrial HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and endoplasmic reticulum (HSP90B1; MIM 191175) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![TRAP1 antibody [N1N3] detects TRAP1 protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded ovarian carcinoma. TRAP1 antibody [N1N3] (GTX115352) dilution: 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min TRAP1 antibody [N1N3] detects TRAP1 protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded ovarian carcinoma. TRAP1 antibody [N1N3] (GTX115352) dilution: 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX115352/GTX115352_40639_IHC_w_23060519_900.webp)
![TRAP1 antibody [N1N3] detects TRAP1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min and permeabilized with 100% MeOH for 30 sec. Green: TRAP1 protein stained by TRAP1 antibody [N1N3] (GTX115352) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm. TRAP1 antibody [N1N3] detects TRAP1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min and permeabilized with 100% MeOH for 30 sec. Green: TRAP1 protein stained by TRAP1 antibody [N1N3] (GTX115352) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX115352/GTX115352_40639_IFA_w_23060519_623.webp)
![Wild-type (WT) and TRAP1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody [N1N3] (GTX115352) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Wild-type (WT) and TRAP1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody [N1N3] (GTX115352) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX115352/GTX115352_40639_20180119_WB_KO_watermark_w_23060519_370.webp)





