IHC-P analysis of human liver tissue using GTX16195 TRAP1 antibody [TRAP1-6]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
![IP analysis of TRAP1 using GTX16195 TRAP1 antibody [TRAP1-6]. The results were visualized by Coomassie Blue staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX16195/GTX16195_2053_IP_w_23060620_634.webp "IP analysis of TRAP1 using GTX16195 TRAP1 antibody [TRAP1-6]. The results were visualized by Coomassie Blue staining.")
![ICC/IF analysis of NCI-H460 Cells using GTX16195 TRAP1 antibody [TRAP1-6]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX16195/GTX16195_349_ICC-IF_w_23060620_967.webp "ICC/IF analysis of NCI-H460 Cells using GTX16195 TRAP1 antibody [TRAP1-6]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of PC-3-M cells using GTX16195 TRAP1 antibody [TRAP1-6].](https://www.genetex.com/upload/website/prouct_img/normal/GTX16195/GTX16195_350_ICC-IF_w_23060620_469.webp "ICC/IF analysis of PC-3-M cells using GTX16195 TRAP1 antibody [TRAP1-6].")
IHC-P analysis of human liver tissue using GTX16195 TRAP1 antibody [TRAP1-6]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
TRAP1 antibody [TRAP1-6]
GTX16195
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetTRAP1
Overview
- SupplierGeneTex
- Product NameTRAP1 antibody [TRAP1-6]
- Delivery Days Customer9
- Antibody SpecificityImmunofluorescence staining of TRAP1 in PC-3-M cells with this antibody produces a pattern consistent with mitochondrial staining.
- Application Supplier NoteWB: 1:2,000. ICC/IF: 1:250. IHC-P: 1:10-1:100. IHC-Fr: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDTRAP1-6
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID10131
- Target nameTRAP1
- Target descriptionTNF receptor associated protein 1
- Target synonymsheat shock protein 75 kDa, mitochondrial; HSP 75; HSP75; HSP90L; testicular tissue protein Li 209; TNFR-associated protein 1; TRAP-1; tumor necrosis factor type 1 receptor-associated protein
- HostMouse
- IsotypeIgG1
- Protein IDQ12931
- Protein NameHeat shock protein 75 kDa, mitochondrial
- Scientific DescriptionThis gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- N-terminal functional domain of Gasdermin A3 regulates mitochondrial homeostasis via mitochondrial targeting. Lin PH et al., 2015 Jun 24, J Biomed SciRead more