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ProductsBack/TRF1 antibody [8A5]
NT2D1 whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TRF1 antibody [8A5] (GTX70290) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
NT2D1 whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TRF1 antibody [8A5] (GTX70290) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
NT2D1 whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TRF1 antibody [8A5] (GTX70290) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

TRF1 antibody [8A5]

GTX70290
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetTERF1
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Overview

  • Supplier
    GeneTex
  • Product Name
    TRF1 antibody [8A5]
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8A5
  • Concentration
    0.1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7013
  • Target name
    TERF1
  • Target description
    telomeric repeat binding factor 1
  • Target synonyms
    PIN2, TRBF1, TRF, TRF1, hTRF1-AS, t-TRF1, telomeric repeat-binding factor 1, NIMA-interacting protein 2, TTAGGG repeat-binding factor 1, telomeric protein Pin2/TRF1, telomeric repeat binding factor (NIMA-interacting) 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP54274
  • Protein Name
    Telomeric repeat-binding factor 1
  • Scientific Description
    This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Huang D, Lu W, Zou S, et al. Rho GDP-dissociation inhibitor α is a potential prognostic biomarker and controls telomere regulation in colorectal cancer. Cancer Sci. 2017,108(7):1293-1302. doi: 10.1111/cas.13259
    Read this paper

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Figure 1. Western blot analysis of TRF1/TERF1 using anti-TRF1/TERF1 antibody (A02474-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRF1/TERF1 antigen affinity purified polyclonal antibody (Catalog # A02474-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRF1/TERF1 at approximately 55 kDa. The expected band size for TRF1/TERF1 is at 50 kDa.
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