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ICC/IF analysis of HeLa cells using GTX10579 TRF1 antibody [TRF-78] at 10 microg/mL(red)with DAPI(blue). Cells were fixed and permeabilized with 4% PFA followed by 0.5% Triton X-100.
ICC/IF analysis of HeLa cells using GTX10579 TRF1 antibody [TRF-78] at 10 microg/mL(red)with DAPI(blue). Cells were fixed and permeabilized with 4% PFA followed by 0.5% Triton X-100.
ICC/IF analysis of HeLa cells using GTX10579 TRF1 antibody [TRF-78] at 10 microg/mL(red)with DAPI(blue). Cells were fixed and permeabilized with 4% PFA followed by 0.5% Triton X-100.

TRF1 antibody [TRF-78]

GTX10579
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, Other Application
Product group Antibodies
ReactivityHuman
TargetTERF1
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Overview

  • Supplier
    GeneTex
  • Product Name
    TRF1 antibody [TRF-78]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 2-4 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    TRF-78
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7013
  • Target name
    TERF1
  • Target description
    telomeric repeat binding factor 1
  • Target synonyms
    PIN2, TRBF1, TRF, TRF1, hTRF1-AS, t-TRF1, telomeric repeat-binding factor 1, NIMA-interacting protein 2, TTAGGG repeat-binding factor 1, telomeric protein Pin2/TRF1, telomeric repeat binding factor (NIMA-interacting) 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP54274
  • Protein Name
    Telomeric repeat-binding factor 1
  • Scientific Description
    TRF1 binds the double-stranded telomeric repeat sequence, TTAGGG.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Watanabe S, Iimori M, Chan DV, et al. MDC1 methylation mediated by lysine methyltransferases EHMT1 and EHMT2 regulates active ATM accumulation flanking DNA damage sites. Sci Rep. 2018,8(1):10888. doi: 10.1038/s41598-018-29239-3
    Read this paper
  • Caslini C, Connelly JA, Serna A, et al. MLL associates with telomeres and regulates telomeric repeat-containing RNA transcription. Mol Cell Biol. 2009,29(16):4519-26. doi: 10.1128/MCB.00195-09
    Read this paper

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Figure 1. Western blot analysis of TRF1/TERF1 using anti-TRF1/TERF1 antibody (A02474-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRF1/TERF1 antigen affinity purified polyclonal antibody (Catalog # A02474-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRF1/TERF1 at approximately 55 kDa. The expected band size for TRF1/TERF1 is at 50 kDa.
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