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TRIM29 Antibody (YA2552, Rabbit)

HY-P82807
MedChem Express
TargetTRIM29
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    TRIM29 Antibody (YA2552, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID23650
  • Target name
    TRIM29
  • Target description
    tripartite motif containing 29
  • Target synonyms
    ATDC, tripartite motif-containing protein 29, ataxia-telangiectasia group D-associated protein, tripartite motif protein TRIM29
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    TRIM29 Antibody (YA2552) is a non-conjugated IgG antibody, targeting TRIM29, with a predicted molecular weight of 66 kDa (observed band size: 66 kDa). TRIM29 Antibody (YA2552) can be used for WB experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRIM29 using anti-TRIM29 antibody (A04472-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM29 antigen affinity purified polyclonal antibody (Catalog # A04472-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM29 at approximately 70 kDa. The expected band size for TRIM29 is at 67 kDa.
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