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ProductsBack/TRPM4 Mouse Monoclonal Antibody [Clone ID: OTI10H5]
Simple Western™ analysis of endogenous protein TRPM4 from Jurkat lysates (0.2 mg/mL) using TRPM4 Mouse Monoclonal Antibody #TA500381. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.
Simple Western™ analysis of endogenous protein TRPM4 from Jurkat lysates (0.2 mg/mL) using TRPM4 Mouse Monoclonal Antibody #TA500381. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.
Simple Western™ analysis of endogenous protein TRPM4 from Jurkat lysates (0.2 mg/mL) using TRPM4 Mouse Monoclonal Antibody #TA500381. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

TRPM4 Mouse Monoclonal Antibody [Clone ID: OTI10H5]

CF500381
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey
TargetTRPM4
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    TRPM4 Mouse Monoclonal Antibody [Clone ID: OTI10H5]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI10H5
  • Gene ID54795
  • Target name
    TRPM4
  • Target description
    transient receptor potential cation channel subfamily M member 4
  • Target synonyms
    EKVP6, LTrpC4, PFHB1B, TRPM4B, hTRPM4, transient receptor potential cation channel subfamily M member 4, calcium-activated non-selective cation channel 1, long transient receptor potential channel 4, melastatin-4
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ8TD43
  • Protein Name
    Transient receptor potential cation channel subfamily M member 4
  • Scientific Description
    Carrier-free (BSA/glycerol-free) TRPM4 mouse monoclonal antibody, clone OTI10H5 (formerly 10H5)
  • Reactivity
    Canine, Human, Monkey
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of TRPM4 using anti-TRPM4 antibody (PB9902). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM4 antigen affinity purified polyclonal antibody (Catalog # PB9902) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM4 at approximately 167 kDa. The expected band size for TRPM4 is at 134 kDa.
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