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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA138687(TRPM5 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA138687(TRPM5 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA138687(TRPM5 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: x200)

TRPM5 Antibody

CSB-PA138687
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTRPM5
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Overview

  • Supplier
    Cusabio
  • Product Name
    TRPM5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29850
  • Target name
    TRPM5
  • Target description
    transient receptor potential cation channel subfamily M member 5
  • Target synonyms
    LTRPC5, MTR1, transient receptor potential cation channel subfamily M member 5, MLSN1 and TRP-related, MLSN1- and TRP-related gene 1 protein, long transient receptor potential channel 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZQ8
  • Protein Name
    Transient receptor potential cation channel subfamily M member 5
  • Scientific Description
    This gene encodes a member of the transient receptor potential (TRP) protein family, which is a diverse group of proteins with structural features typical of ion channels. This protein plays an important role in taste transduction, and has characteristics of a calcium-activated, non-selective cation channel that carries Na+, K+, and Cs+ ions equally well, but not Ca(2+) ions. It is activated by lower concentrations of intracellular Ca(2+), and inhibited by higher concentrations. It is also a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. This gene is located within the Beckwith-Wiedemann syndrome critical region-1 on chromosome 11p15.5, and has been shown to be imprinted, with exclusive expression from the paternal allele.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRPM5 using anti-TRPM5 antibody (PB9903). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SMMC whole cell lysates, Lane 2: 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM5 antigen affinity purified polyclonal antibody (Catalog # PB9903) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM5 at approximately 131 kDa. The expected band size for TRPM5 is at 131 kDa.
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