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Lane 1: Human Liver cell lysates probed with TRPM5 Polyclonal Antibody, Unconjugated (bs-9047R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Liver cell lysates probed with TRPM5 Polyclonal Antibody, Unconjugated (bs-9047R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Liver cell lysates probed with TRPM5 Polyclonal Antibody, Unconjugated (bs-9047R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

TRPM5 Polyclonal Antibody

BS-9047R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat
TargetTRPM5
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Overview

  • Supplier
    Bioss
  • Product Name
    TRPM5 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IF(IHC-P)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID29850
  • Target name
    TRPM5
  • Target description
    transient receptor potential cation channel subfamily M member 5
  • Target synonyms
    LTRPC5, MTR1, transient receptor potential cation channel subfamily M member 5, MLSN1 and TRP-related, MLSN1- and TRP-related gene 1 protein, long transient receptor potential channel 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Bovine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRPM5 using anti-TRPM5 antibody (PB9903). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SMMC whole cell lysates, Lane 2: 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM5 antigen affinity purified polyclonal antibody (Catalog # PB9903) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM5 at approximately 131 kDa. The expected band size for TRPM5 is at 131 kDa.
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