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TRPM5 Antibody

LS-C402832
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTRPM5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TRPM5 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:50 - 1:200), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.6 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID29850
  • Target name
    TRPM5
  • Target description
    transient receptor potential cation channel subfamily M member 5
  • Target synonyms
    LTRPC5, MTR1, transient receptor potential cation channel subfamily M member 5, MLSN1 and TRP-related, MLSN1- and TRP-related gene 1 protein, long transient receptor potential channel 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRPM5 using anti-TRPM5 antibody (PB9903). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SMMC whole cell lysates, Lane 2: 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM5 antigen affinity purified polyclonal antibody (Catalog # PB9903) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM5 at approximately 131 kDa. The expected band size for TRPM5 is at 131 kDa.
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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA138687(TRPM5 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: x200)
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