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ProductsBack/TRPS1 antibody [MSVA-512R] HistoMAX(tm)
IHC-P analysis of human invasive breast cancer of no special type (NST) tissue using GTX04485 TRPS1 antibody [MSVA-512R] HistoMAX?. Invasive breast cancer of no special type NST with intense nuclear TRPS1 positivity.
IHC-P analysis of human invasive breast cancer of no special type (NST) tissue using GTX04485 TRPS1 antibody [MSVA-512R] HistoMAX?. Invasive breast cancer of no special type NST with intense nuclear TRPS1 positivity.
IHC-P analysis of human invasive breast cancer of no special type (NST) tissue using GTX04485 TRPS1 antibody [MSVA-512R] HistoMAX?. Invasive breast cancer of no special type NST with intense nuclear TRPS1 positivity.

TRPS1 antibody [MSVA-512R] HistoMAX(tm)

GTX04485
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTRPS1
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Overview

  • Supplier
    GeneTex
  • Product Name
    TRPS1 antibody [MSVA-512R] HistoMAX(tm)
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:100-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MSVA-512R
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7227
  • Target name
    TRPS1
  • Target description
    transcriptional repressor GATA binding 1
  • Target synonyms
    GC79, LGCR, zinc finger transcription factor Trps1, tricho-rhino-phalangeal syndrome type I protein, trichorhinophalangeal syndrome I, zinc finger protein GC79
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UHF7
  • Protein Name
    Zinc finger transcription factor Trps1
  • Scientific Description
    This gene encodes a transcription factor that represses GATA-regulated genes and binds to a dynein light chain protein. Binding of the encoded protein to the dynein light chain protein affects binding to GATA consensus sequences and suppresses its transcriptional activity. Defects in this gene are a cause of tricho-rhino-phalangeal syndrome (TRPS) types I-III. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRPS1 using anti-TRPS1 antibody (A02328-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPS1 antigen affinity purified polyclonal antibody (Catalog # A02328-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPS1 at approximately 150 kDa. The expected band size for TRPS1 is at 142 kDa.
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