![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638190/GTX638190_T-44935_20230203_WB_TPM_watermark_23020621_688.webp)
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Tryptophanyl tRNA synthetase antibody [HL2186]
GTX638190
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetWARS1
Overview
- SupplierGeneTex
- Product NameTryptophanyl tRNA synthetase antibody [HL2186]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2186
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7453
- Target nameWARS1
- Target descriptiontryptophanyl-tRNA synthetase 1
- Target synonymsGAMMA-2, HMN9, HMND9, IFI53, IFP53, NEDMSBA, WARS, tryptophan--tRNA ligase, cytoplasmic, epididymis secretory sperm binding protein, hWRS, interferon-induced protein 53, trpRS, tryptophan tRNA ligase 1, cytoplasmic
- HostRabbit
- IsotypeIgG
- Protein IDP23381
- Protein NameTryptophan--tRNA ligase, cytoplasmic
- Scientific DescriptionAminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638190/GTX638190_T-44935_20230203_WB_shRNA_watermark_23020621_983.webp)
![Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638190/GTX638190_T-44935_20230203_WB_M_lung_23020621_966.webp)
![Tryptophanyl tRNA synthetase antibody [HL2186] detects Tryptophanyl tRNA synthetase protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Tryptophanyl tRNA synthetase stained by Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Tryptophanyl tRNA synthetase antibody [HL2186] detects Tryptophanyl tRNA synthetase protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Tryptophanyl tRNA synthetase stained by Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638190/GTX638190_T-44935_20230217_IHC-P_R_23030219_439.webp)
![Tryptophanyl tRNA synthetase antibody [HL2186] detects Tryptophanyl tRNA synthetase protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded mouse adrenal gland. Tryptophanyl tRNA synthetase stained by Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Tryptophanyl tRNA synthetase antibody [HL2186] detects Tryptophanyl tRNA synthetase protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded mouse adrenal gland. Tryptophanyl tRNA synthetase stained by Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638190/GTX638190_T-44935_20230217_IHC-P_M_23030219_688.webp)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638190/GTX638190_45005_20230414_WB_TPM_watermark_23041723_360.webp)
![Tryptophanyl tRNA synthetase antibody [HL2186] detects Tryptophanyl tRNA synthetase protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Tryptophanyl tRNA synthetase stained by Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Tryptophanyl tRNA synthetase antibody [HL2186] detects Tryptophanyl tRNA synthetase protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Tryptophanyl tRNA synthetase stained by Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638190/GTX638190_45005_20230512_ICC_IF_23060622_371.webp)
![Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638190/GTX638190_45005_20230609_WB_Z_23061400_423.webp)






