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TUBA1A Recombinant Antibody AE00319

AE00319
Aeonian Biotech
TargetTUBA1A
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Product group Antibodies
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Overview

  • Supplier
    Aeonian Biotech
  • Product Name
    TUBA1A Recombinant Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    ICC, WB
  • Category Supplier
    Antibody
  • Certification
    Research Use Only
  • Clonality
    Recombinant
  • Clone ID
    rF2C
  • Conjugate
    Unconjugated
  • Gene ID7846
  • Target name
    TUBA1A
  • Target description
    tubulin alpha 1a
  • Target synonyms
    B-ALPHA-1; hum-a-tub1; hum-a-tub2; LIS3; TUBA3; tubulin alpha-1A chain; tubulin alpha-3 chain; tubulin B-alpha-1; tubulin, alpha, brain-specific
  • Host
    Rat
  • Isotype
    IgG lambda
  • Protein IDQ71U36
  • Protein Name
    Tubulin alpha-1A chain
  • Scientific Description
    TUBA1A Recombinant Antibody AE00319
  • Shelf life instruction
    Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
  • Reactivity Supplier
    Human
  • Storage Instruction
    2-8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Tubulin alpha using anti-Tubulin alpha antibody (A03989-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tubulin alpha antigen affinity purified polyclonal antibody (Catalog # A03989-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tubulin alpha at approximately 50 kDa. The expected band size for Tubulin alpha is at 50 kDa.
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Confocal microscopy of PFA-fixed and 0.15% Triton X-100 permealised HeLa cells stained with TUBA1A Rabbit Recombinant Antibody AE00320 at 10ug/ml for 1h at RT. Detection by CF488 (green) for the antibody and DAPI (blue) for nuclear staining. Bottom right shows staining with an isotype control antibody.
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