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Confocal microscopy of PFA-fixed and 0.15% Triton X-100 permealised HeLa cells stained with TUBA1A Rabbit Recombinant Antibody AE00320 at 10ug/ml for 1h at RT. Detection by CF488 (green) for the antibody and DAPI (blue) for nuclear staining. Bottom right shows staining with an isotype control antibody.
Confocal microscopy of PFA-fixed and 0.15% Triton X-100 permealised HeLa cells stained with TUBA1A Rabbit Recombinant Antibody AE00320 at 10ug/ml for 1h at RT. Detection by CF488 (green) for the antibody and DAPI (blue) for nuclear staining. Bottom right shows staining with an isotype control antibody.
Confocal microscopy of PFA-fixed and 0.15% Triton X-100 permealised HeLa cells stained with TUBA1A Rabbit Recombinant Antibody AE00320 at 10ug/ml for 1h at RT. Detection by CF488 (green) for the antibody and DAPI (blue) for nuclear staining. Bottom right shows staining with an isotype control antibody.

TUBA1A Recombinant Antibody AE00320

AE00320
Aeonian Biotech
TargetTUBA1A
Product group Antibodies
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Overview

  • Supplier
    Aeonian Biotech
  • Product Name
    TUBA1A Recombinant Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    ICC, IHC, WB
  • Category Supplier
    Antibody
  • Certification
    Research Use Only
  • Clonality
    Recombinant
  • Clone ID
    F2CR
  • Conjugate
    Unconjugated
  • Gene ID7846
  • Target name
    TUBA1A
  • Target description
    tubulin alpha 1a
  • Target synonyms
    B-ALPHA-1; hum-a-tub1; hum-a-tub2; LIS3; TUBA3; tubulin alpha-1A chain; tubulin alpha-3 chain; tubulin B-alpha-1; tubulin, alpha, brain-specific
  • Host
    Rabbit
  • Isotype
    IgG lambda
  • Protein IDQ71U36
  • Protein Name
    Tubulin alpha-1A chain
  • Scientific Description
    TUBA1A Recombinant Antibody AE00320
  • Shelf life instruction
    Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
  • Reactivity Supplier
    Human
  • Storage Instruction
    2-8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Tubulin alpha using anti-Tubulin alpha antibody (A03989-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tubulin alpha antigen affinity purified polyclonal antibody (Catalog # A03989-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tubulin alpha at approximately 50 kDa. The expected band size for Tubulin alpha is at 50 kDa.
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