Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody (GTX101763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![TUFM antibody detects TUFM protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TUFM stained by TUFM antibody (GTX101763) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX101763/GTX101763_44714_20220916_ICC_IF_22102723_868.webp "TUFM antibody detects TUFM protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TUFM stained by TUFM antibody (GTX101763) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
![TUFM antibody immunoprecipitates TUFM protein in IP experiments. IP samples: HepG2 whole cell extract A. 35 μg HepG2 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of TUFM protein by 4 μg TUFM antibody (GTX101763) 10 % SDS-PAGE The immunoprecipitated TUFM protein was detected by TUFM antibody (GTX101763) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX101763/GTX101763_39694_IP_w_23060100_823.webp "TUFM antibody immunoprecipitates TUFM protein in IP experiments. IP samples: HepG2 whole cell extract A. 35 μg HepG2 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of TUFM protein by 4 μg TUFM antibody (GTX101763) 10 % SDS-PAGE The immunoprecipitated TUFM protein was detected by TUFM antibody (GTX101763) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]")
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody (GTX101763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
TUFM antibody
GTX101763
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetTUFM
Overview
- SupplierGeneTex
- Product NameTUFM antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.3 mg/ml
- ConjugateUnconjugated
- Gene ID7284
- Target nameTUFM
- Target descriptionTu translation elongation factor, mitochondrial
- Target synonymsCOXPD4, EF-TuMT, EFTU, P43, elongation factor Tu, mitochondrial, EF-Tu, epididymis secretory sperm binding protein
- HostRabbit
- IsotypeIgG
- Protein IDP49411
- Protein NameElongation factor Tu, mitochondrial
- Scientific DescriptionThis gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Avolio R, Agliarulo I, Criscuolo D, et al. Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins. Genome Res. 2023,33(8):1242-1257. doi: 10.1101/gr.277755.123Read this paper
- Avolio R, Agliarulo I, Criscuolo D, et al. Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins. bioRxiv. 2023,:pii: 2023.01.19.524708. doi: 10.1101/2023.01.19.524708.Read this paper
- Sundararaman B, Zhan L, Blue SM, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016,61(6):903-13. doi: 10.1016/j.molcel.2016.02.012Read this paper
Datasheet
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