
Immunohistochemical analysis of paraffin-embedded SNU16 xenograft, using TUFM(GTX101764) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
TUFM antibody [N3C3]
GTX101764
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityHuman
TargetTUFM
Overview
- SupplierGeneTex
- Product NameTUFM antibody [N3C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7284
- Target nameTUFM
- Target descriptionTu translation elongation factor, mitochondrial
- Target synonymsCOXPD4, EF-TuMT, EFTU, P43, elongation factor Tu, mitochondrial, EF-Tu, epididymis secretory sperm binding protein
- HostRabbit
- IsotypeIgG
- Protein IDP49411
- Protein NameElongation factor Tu, mitochondrial
- Scientific DescriptionThis gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![TUFM antibody [N3C3] immunoprecipitates TUFM protein in IP experiments. IP samples: HepG2 whole cell extract A. 35 μg HepG2 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of TUFM protein by 4 μg TUFM antibody [N3C3] (GTX101764) 10 % SDS-PAGE The immunoprecipitated TUFM protein was detected by TUFM antibody [N3C3] (GTX101764) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent] TUFM antibody [N3C3] immunoprecipitates TUFM protein in IP experiments. IP samples: HepG2 whole cell extract A. 35 μg HepG2 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of TUFM protein by 4 μg TUFM antibody [N3C3] (GTX101764) 10 % SDS-PAGE The immunoprecipitated TUFM protein was detected by TUFM antibody [N3C3] (GTX101764) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX101764/GTX101764_40324_IP_w_23060100_628.webp)






![Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TUFM antibody [HL2117] (GTX638089) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638089/GTX638089_T-44914_20230113_WB_M_heart_23011618_997.webp)
![TUFM antibody [HL2193] detects TUFM protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TUFM stained by TUFM antibody [HL2193] (GTX638197) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638197/GTX638197_T-44942_20230331_ICC_IF_23041023_194.webp)
