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TWIST1

ORB153387
Biorbyt
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetTWIST1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    TWIST1
  • Delivery Days Customer
    16
  • Applications
    ELISA
  • Applications Supplier
    ELISA: 1/10000 ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2F8E7
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7291
  • Target name
    TWIST1
  • Target description
    twist family bHLH transcription factor 1
  • Target synonyms
    ACS3, BPES2, BPES3, CRS, CRS1, CSO, SCS, SWCOS, TWIST, bHLHa38, twist-related protein 1, B-HLH DNA binding protein, H-twist, TWIST homolog of drosophila, class A basic helix-loop-helix protein 38, twist basic helix-loop-helix transcription factor 1, twist homolog 1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ15672
  • Protein Name
    Twist-related protein 1
  • Scientific Description
    Mouse monoclonal antibody to TWIST1
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TWIST1/2 using anti-TWIST1/2 antibody (A00980-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat ovary tissue lysate, Lane 3: mouse brain tissue lysate, Lane 4: mouse ovary tissue lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWIST1/2 antigen affinity purified polyclonal antibody (Catalog # A00980-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TWIST1/2 at approximately 30 kDa. The expected band size for TWIST1/2 is at 21 kDa.
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Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: TWIST1 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21 kDa Observed band size: 50 kDa
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