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ProductsBack/TYRO3 Mouse Monoclonal Antibody [Clone ID: OTI5B4]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TYRO3 (Cat# RC208260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3(Cat# TA500413). Positive lysates [LY401899] (100ug) and [LC401899] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TYRO3 (Cat# RC208260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3(Cat# TA500413). Positive lysates [LY401899] (100ug) and [LC401899] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TYRO3 (Cat# RC208260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3(Cat# TA500413). Positive lysates [LY401899] (100ug) and [LC401899] (20ug) can be purchased separately from OriGene.

TYRO3 Mouse Monoclonal Antibody [Clone ID: OTI5B4]

CF500413
OriGene
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTYRO3
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    TYRO3 Mouse Monoclonal Antibody [Clone ID: OTI5B4]
  • Delivery Days Customer
    14
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5B4
  • Gene ID7301
  • Target name
    TYRO3
  • Target description
    TYRO3 protein tyrosine kinase
  • Target synonyms
    BYK, Dtk, Etk-2, RSE, Rek, Sky, Tif, tyrosine-protein kinase receptor TYRO3, tyrosine-protein kinase DTK, tyrosine-protein kinase RSE, tyrosine-protein kinase SKY, tyrosine-protein kinase TIF, tyrosine-protein kinase byk
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ06418
  • Protein Name
    Tyrosine-protein kinase receptor TYRO3
  • Scientific Description
    Carrier-free (BSA/glycerol-free) TYRO3 mouse monoclonal antibody, clone OTI5B4 (formerly 5B4)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of TYRO3 using anti-TYRO3 antibody (M00913-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TYRO3 antigen affinity purified monoclonal antibody (Catalog # M00913-5) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TYRO3 at approximately 120 kDa. The expected band size for TYRO3 is at 97 kDa.
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