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Immunohistochemical analysis of paraffin-embedded human brain tissue using PGP9.5 antibody.
Immunohistochemical analysis of paraffin-embedded human brain tissue using PGP9.5 antibody.
Immunohistochemical analysis of paraffin-embedded human brain tissue using PGP9.5 antibody.

UCHL1 Antibody

CSB-PA869556
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetUCHL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    UCHL1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7345
  • Target name
    UCHL1
  • Target description
    ubiquitin C-terminal hydrolase L1
  • Target synonyms
    HEL-117, HEL-S-53, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, SPG79, SPG79A, UCHL-1, Uch-L1, ubiquitin carboxyl-terminal hydrolase isozyme L1, epididymis luminal protein 117, epididymis secretory protein Li 53, neuron cytoplasmic protein 9.5, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase), ubiquitin thioesterase L1, ubiquitin thiolesterase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09936
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase isozyme L1
  • Scientific Description
    Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Diana L. Mandelker, Cancer Res., Jun 2005; 65: 4963 - 4968. Taiji Yamazaki, Clin. Cancer Res., Jan 2002; 8: 192. Ekmel Tezel, Clin. Cancer Res., Dec 2000; 6: 4764. Kenji Hibi, Am. J. Pathol., Sep 1999; 155: 711.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PGP9.5 using anti-PGP9.5 antibody (PB9840). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGP9.5 antigen affinity purified polyclonal antibody (Catalog # PB9840) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGP9.5 at approximately 25 kDa. The expected band size for PGP9.5 is at 25 kDa.
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