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UGT1A / UGT1A1 Antibody (aa251-300)

LS-C40468
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetUGT1A1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    UGT1A / UGT1A1 Antibody (aa251-300)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (2.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID54658
  • Target name
    UGT1A1
  • Target description
    UDP glucuronosyltransferase family 1 member A1
  • Target synonyms
    BILIQTL1, GNT1, HUG-BR1, UDPGT, UDPGT 1-1, UGT1, UGT1A, UDP-glucuronosyltransferase 1A1, UDP glucuronosyltransferase 1 family, polypeptide A1, UDP glycosyltransferase 1 family, polypeptide A1, UDP-glucuronosyltransferase 1-A, UDP-glucuronosyltransferase 1A, UGT-1A, UGT1*1, UGT1-01, UGT1.1, bilirubin UDP-glucuronosyltranserase, bilirubin UDP-glucuronosyltransferase 1-1, bilirubin UDP-glucuronosyltransferase isozyme 1, bilirubin-specific UDPGT isozyme 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of UGT1A1 using anti-UGT1A1 antibody (A01865-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UGT1A1 antigen affinity purified polyclonal antibody (Catalog # A01865-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UGT1A1 at approximately 59KD. The expected band size for UGT1A1 is at 59KD.
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