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Western Blot analysis of Jurkat cells using UHRF1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using UHRF1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using UHRF1 Polyclonal Antibody

UHRF1 Antibody

CSB-PA004690
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetUHRF1
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Overview

  • Supplier
    Cusabio
  • Product Name
    UHRF1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29128
  • Target name
    UHRF1
  • Target description
    ubiquitin like with PHD and ring finger domains 1
  • Target synonyms
    E3 ubiquitin-protein ligase UHRF1; hNP95; hUHRF1; huNp95; ICBP90; inverted CCAAT box-binding protein of 90 kDa; Np95; nuclear phosphoprotein 95; nuclear protein 95; nuclear zinc finger protein Np95; RING finger protein 106; RING-type E3 ubiquitin transferase UHRF1; RNF106; TDRD22; transcription factor ICBP90; ubiquitin-like PHD and RING finger domain-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96T88
  • Protein Name
    E3 ubiquitin-protein ligase UHRF1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human cerebral cortex, liver, lymph node and thymus using Anti-UHRF1 antibody HPA049408 (A) shows similar protein distribution across tissues to independent antibody HPA055446 (B).
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Figure 1. Western blot analysis of UHRF1 using anti-UHRF1 antibody (A01156-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UHRF1 antigen affinity purified polyclonal antibody (Catalog # A01156-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UHRF1 at approximately 100 kDa. The expected band size for UHRF1 is at 90 kDa.
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