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UHRF1 Polyclonal Antibody

BS-25668R
Bioss Antibodies
ApplicationsImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetUHRF1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    UHRF1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID29128
  • Target name
    UHRF1
  • Target description
    ubiquitin like with PHD and ring finger domains 1
  • Target synonyms
    E3 ubiquitin-protein ligase UHRF1; hNP95; hUHRF1; huNp95; ICBP90; inverted CCAAT box-binding protein of 90 kDa; Np95; nuclear phosphoprotein 95; nuclear protein 95; nuclear zinc finger protein Np95; RING finger protein 106; RING-type E3 ubiquitin transferase UHRF1; RNF106; TDRD22; transcription factor ICBP90; ubiquitin-like PHD and RING finger domain-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96T88
  • Protein Name
    E3 ubiquitin-protein ligase UHRF1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UHRF1 using anti-UHRF1 antibody (A01156-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UHRF1 antigen affinity purified polyclonal antibody (Catalog # A01156-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UHRF1 at approximately 100 kDa. The expected band size for UHRF1 is at 90 kDa.
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