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ULBP1 antibody [N1C3]

GTX123021
GeneTex
ApplicationsFunctional Assay, Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetULBP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ULBP1 antibody [N1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Functional Assay, Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID80329
  • Target name
    ULBP1
  • Target description
    UL16 binding protein 1
  • Target synonyms
    N2DL-1, NKG2DL1, RAET1I, UL16-binding protein 1, NKG2D ligand 1, alcan-beta, retinoic acid early transcript 1I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BZM6
  • Protein Name
    UL16-binding protein 1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • ULBP1 is induced by hepatitis C virus infection and is the target of the NK cell-mediated innate immune response in human hepatocytes. Dansako H et al., 2018 Mar, FEBS Open Bio
    Read more

Datasheet

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Figure 1. Western blot analysis of ULBP1 using anti-ULBP1 antibody (A04323-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ULBP1 antigen affinity purified polyclonal antibody (Catalog # A04323-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ULBP1 at approximately 28 kDa. The expected band size for ULBP1 is at 28 kDa.
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