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UQCRH Rabbit mAb

A9395
ABclonal
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetUQCRH
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Overview

  • Supplier
    ABclonal
  • Product Name
    UQCRH Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID7388
  • Target name
    UQCRH
  • Target description
    ubiquinol-cytochrome c reductase hinge protein
  • Target synonyms
    MC3DN11, QCR6, UQCR8, cytochrome b-c1 complex subunit 6, mitochondrial, complex III subunit 6, cytochrome c1 non-heme 11 kDa protein, mitochondrial hinge protein, ubiquinol-cytochrome c reductase complex 11 kDa protein, ubiquinol-cytochrome c reductase, complex III subunit VIII
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07919
  • Protein Name
    Cytochrome b-c1 complex subunit 6, mitochondrial
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of UQCRH using anti-UQCRH antibody (M09436). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UQCRH antigen affinity purified monoclonal antibody (Catalog # M09436) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UQCRH at approximately 13 kDa. The expected band size for UQCRH is at 11 kDa.
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