IHC-P analysis of human colon adenocarcinoma tissue using GTX83431 USP5 antibody [2F5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
![IHC-P analysis of human kidney tissue using GTX83431 USP5 antibody [2F5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83431/GTX83431_1369_IHC-P_w_23061419_401.webp "IHC-P analysis of human kidney tissue using GTX83431 USP5 antibody [2F5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of human prostate tissue using GTX83431 USP5 antibody [2F5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83431/GTX83431_1370_IHC-P_w_23061419_415.webp "IHC-P analysis of human prostate tissue using GTX83431 USP5 antibody [2F5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of human thyroid tissue using GTX83431 USP5 antibody [2F5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83431/GTX83431_1371_IHC-P_w_23061419_210.webp "IHC-P analysis of human thyroid tissue using GTX83431 USP5 antibody [2F5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![WB analysis of various cell lines using GTX83431 USP5 antibody [2F5]. Loading : 35 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX83431/GTX83431_3416_WB_w_23061419_467.webp "WB analysis of various cell lines using GTX83431 USP5 antibody [2F5]. Loading : 35 ug per lane Dilution : 1:200")
![WB analysis of HEK293T cells transfected with USP5 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83431 USP5 antibody [2F5]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83431/GTX83431_3624_WB_w_23061419_762.webp "WB analysis of HEK293T cells transfected with USP5 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83431 USP5 antibody [2F5]. Loading : 5 ug per lane")
![ICC/IF analysis of COS7 cells transiently transfected with USP5 plasmid using GTX83431 USP5 antibody [2F5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83431/GTX83431_668_ICCIF_w_23061419_469.webp "ICC/IF analysis of COS7 cells transiently transfected with USP5 plasmid using GTX83431 USP5 antibody [2F5].")
IHC-P analysis of human colon adenocarcinoma tissue using GTX83431 USP5 antibody [2F5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
USP5 antibody [2F5]
GTX83431
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetUSP5
Overview
- SupplierGeneTex
- Product NameUSP5 antibody [2F5] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:2000. ICC/IF: 1:100. IHC-P: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2F5
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID8078
- Target nameUSP5
- Target descriptionubiquitin specific peptidase 5
- Target synonymsdeubiquitinating enzyme 5; isopeptidase T; ISOT; testicular tissue protein Li 218; ubiquitin carboxyl-terminal hydrolase 5; ubiquitin isopeptidase T; ubiquitin specific protease 5 (isopeptidase T); ubiquitin thioesterase 5; ubiquitin thiolesterase 5; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); ubiquitin-specific-processing protease 5
- HostMouse
- IsotypeIgG1
- Protein IDP45974
- Protein NameUbiquitin carboxyl-terminal hydrolase 5
- Scientific DescriptionCleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored Lys-48-linked polyubiquitin disassembly. Binds linear and Lys-63-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition.
- ReactivityCanine, Human, Monkey, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203