Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with USP7 antibody [GT481] (GTX631108) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
![Immunoprecipitation of USP7 protein from HepG2 whole cell extracts using 5 μg of USP7 antibody [GT481] (GTX631108). Western blot analysis was performed using USP7 antibody [GT481] (GTX631108). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631108/GTX631108_41688_20150717_IP_w_23061202_352.webp "Immunoprecipitation of USP7 protein from HepG2 whole cell extracts using 5 μg of USP7 antibody [GT481] (GTX631108). Western blot analysis was performed using USP7 antibody [GT481] (GTX631108). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.")
![USP7 antibody [GT481] detects USP7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: USP7 stained by USP7 antibody [GT481] (GTX631108) diluted at 1:500. Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000. Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631108/GTX631108_44517_20220408_ICC_IF_w_23061202_452.webp "USP7 antibody [GT481] detects USP7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: USP7 stained by USP7 antibody [GT481] (GTX631108) diluted at 1:500. Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000. Scale bar= 10μm.")
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with USP7 antibody [GT481] (GTX631108) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
USP7 antibody [GT481]
GTX631108
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetUSP7
Overview
- SupplierGeneTex
- Product NameUSP7 antibody [GT481]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT481
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7874
- Target nameUSP7
- Target descriptionubiquitin specific peptidase 7
- Target synonymsC16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1, ubiquitin carboxyl-terminal hydrolase 7, Chromosome 16p13.2 deletion syndrome, Herpes virus-associated ubiquitin-specific protease, deubiquitinating enzyme 7, ubiquitin specific peptidase 7 (herpes virus-associated), ubiquitin specific protease 7 (herpes virus-associated), ubiquitin thioesterase 7, ubiquitin-specific-processing protease 7
- HostMouse
- IsotypeIgG2a
- Protein IDQ93009
- Protein NameUbiquitin carboxyl-terminal hydrolase 7
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Wang Y, Yang L, Zhang X, et al. Epigenetic regulation of ferroptosis by H2B monoubiquitination and p53. EMBO Rep. 2019,20(7):e47563. doi: 10.15252/embr.201847563Read this paper
- Xiang Q, Ju H, Li Q, et al. Human Herpesvirus 8 Interferon Regulatory Factors 1 and 3 Mediate Replication and Latency Activities via Interactions with USP7 Deubiquitinase. J Virol. 2018,92(7). doi: 10.1128/JVI.02003-17
Datasheet
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