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USP7 antibody detects USP7 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: USP7 stained by USP7 antibody (GTX125894) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.
USP7 antibody detects USP7 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: USP7 stained by USP7 antibody (GTX125894) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.
USP7 antibody detects USP7 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: USP7 stained by USP7 antibody (GTX125894) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.

USP7 antibody

GTX125894
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetUSP7
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Overview

  • Supplier
    GeneTex
  • Product Name
    USP7 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.34 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7874
  • Target name
    USP7
  • Target description
    ubiquitin specific peptidase 7
  • Target synonyms
    C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1, ubiquitin carboxyl-terminal hydrolase 7, Chromosome 16p13.2 deletion syndrome, Herpes virus-associated ubiquitin-specific protease, deubiquitinating enzyme 7, ubiquitin specific peptidase 7 (herpes virus-associated), ubiquitin specific protease 7 (herpes virus-associated), ubiquitin thioesterase 7, ubiquitin-specific-processing protease 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ93009
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase 7
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 2. IHC analysis of HAUSP/USP7 using anti-HAUSP/USP7 antibody (A01239-2). HAUSP/USP7 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-HAUSP/USP7 Antibody (A01239-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Western blot using GeneTex affinity purified anti-HAUSP antibody (GTX24080) shows detection of HAUSP in various cell lysates at 130 kDa (lane 1 - HeLa nuclear extract, lane 2 - HeLa, Lane 3 - A431, Lane 4 - MCF7, Lane 5 - 3T3). The antibody is blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates. A 1:500 dilution of the primary antibody was used for detection followed by a 1:5,000 dilution of HRP Gt-a-Rabbit IgG. Exposure time was 30 s.
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