Western blot using GeneTex affinity purified anti-HAUSP antibody (GTX24080) shows detection of HAUSP in various cell lysates at 130 kDa (lane 1 - HeLa nuclear extract, lane 2 - HeLa, Lane 3 - A431, Lane 4 - MCF7, Lane 5 - 3T3). The antibody is blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates. A 1:500 dilution of the primary antibody was used for detection followed by a 1:5,000 dilution of HRP Gt-a-Rabbit IgG. Exposure time was 30 s.

USP7 antibody

GTX24080

ApplicationsWestern Blot, ELISA

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Product group Antibodies

TargetUSP7

Overview

Supplier
GeneTex
Product Name
USP7 antibody
Delivery Days Customer
9
Application Supplier Note
WB: 1:500-1:2000. ELISA: 1:15000-1:60000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Applications
Western Blot, ELISA
Certification
Research Use Only
Clonality
Polyclonal
Concentration
1.1 mg/ml
Conjugate
Unconjugated
Gene ID7874
Target name
USP7
Target description
ubiquitin specific peptidase 7
Target synonyms
deubiquitinating enzyme 7; HAFOUS; HAUSP; Herpes virus-associated ubiquitin-specific protease; TEF1; ubiquitin carboxyl-terminal hydrolase 7; ubiquitin specific peptidase 7 (herpes virus-associated); ubiquitin specific protease 7 (herpes virus-associated); ubiquitin thioesterase 7; ubiquitin-specific-processing protease 7
Host
Rabbit
Isotype
IgG
Protein IDQ93009
Protein Name
Ubiquitin carboxyl-terminal hydrolase 7
Scientific Description
HAUSP is a novel p53 interacting protein identified by mass spectrometry of affinity-purified p53 associated factors by Li et al. HAUSP strongly stabilizes p53 even in the presence of excess MDM2, and also induces p53-dependent cell growth repression and apoptosis. HAUSP has an intrinsic enzymatic activity that specifically de-ubiquitinates p53 both in vivo and in vitro. Expression of a catalytically inactive point mutation of HAUSP in cells increased the levels of p53 ubiquitination and also destabilized p53. Li et al concluded that their findings revealed an important mechanism by which p53 can be stabilized by direct de-ubiquitination and also implied that HAUSP may function as a tumor suppressor in vivo through the stabilization of p53.
Storage Instruction
-20°C or -80°C,2°C to 8°C
UNSPSC
12352203

USP7 antibody

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