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Anti-VCP Antibody

ORB107641
Biorbyt
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetVCP
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Overview

  • Supplier
    Biorbyt
  • Product Name
    VCP Antibody
  • Delivery Days Customer
    16
  • Application Supplier Note
    Western blot, 0.1-0.5microg/ml, Human, Monkey, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1microg/ml, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1microg/ml, Rat Immunocytochemistry , 0.5-1microg/ml, Human Immunocytochemistry/Immunofluorescence, 5microg/ml, Human Flow Cytometry (Fixed), 1-3microg/1x106 cells, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    FC, ICC, IF, IHC, IHC-Fr, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID7415
  • Target name
    VCP
  • Target description
    valosin containing protein
  • Target synonyms
    15S Mg(2+)-ATPase p97 subunit; CDC48; FTDALS6; p97; TER ATPase; TERA; transitional endoplasmic reticulum ATPase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55072
  • Protein Name
    Transitional endoplasmic reticulum ATPase
  • Scientific Description
    Anti-VCP Antibody
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VCP using anti-VCP antibody (A00610-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: Monkey COS-7 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human placenta tissue lysates, Lane 8: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCP antigen affinity purified polyclonal antibody (Catalog # A00610-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCP at approximately 97 kDa. The expected band size for VCP is at 89 kDa.
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