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Western Blot analysis of K562 cells using VDAC1 Polyclonal Antibody.
Western Blot analysis of K562 cells using VDAC1 Polyclonal Antibody.
Western Blot analysis of K562 cells using VDAC1 Polyclonal Antibody.

VDAC1 Antibody

CSB-PA005626
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVDAC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    VDAC1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7416
  • Target name
    VDAC1
  • Target description
    voltage dependent anion channel 1
  • Target synonyms
    PORIN, VDAC-1, non-selective voltage-gated ion channel VDAC1, outer mitochondrial membrane protein porin 1, plasmalemmal porin, porin 31HL, porin 31HM, sperm binding protein 1a
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21796
  • Protein Name
    Voltage-dependent anion-selective channel protein 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody (A01168-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Porin/VDAC1 antigen affinity purified polyclonal antibody (Catalog # A01168-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Porin/VDAC1 at approximately 34 kDa. The expected band size for Porin/VDAC1 is at 31 kDa.
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